GENOMIC SURVEILLANCE OF ANTIMICROBIAL RESISTANCE GENES AND RESISTANCE CLASS DISTRIBUTION IN BACTERIAL ISOLATES
Abstract
Background: Antimicrobial resistance (AMR) is a major global health challenge that threatens the effectiveness of antibiotics used to treat bacterial infections. The increasing prevalence of resistant pathogens highlights the need for genomic surveillance to understand the distribution and evolution of AMR genes in bacterial populations.
Methods: Genomic records of bacterial isolates were analysed to evaluate AMR gene distribution and resistance class diversity. 50 bacterial genomes were examined for genomic characteristics, including genome length and GC content. Resistance genes were identified and categorised into AMR classes. Resistance burden was assessed using total AMR gene counts and resistance class diversity, supported by descriptive statistical analysis and graphical visualisation.
Results: The analysis revealed widespread occurrence of AMR genes across bacterial genomes, with variation in resistance gene counts and resistance class diversity among isolates. Genomes contained multiple resistance determinants linked to different antimicrobial classes, suggesting potential multidrug resistance. Genomes with larger genetic content tended to contain higher numbers of resistance genes.
Conclusion: The findings demonstrate that bacterial genomes harbour diverse resistance determinants and multiple resistance classes, emphasising the importance of genomic surveillance in monitoring resistance evolution and supportingstrategies to control the global spread of AMR.
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