PHARMACOLOGICAL AND THERAPEUTIC POTENTIAL OF ALOE SPECIES: A SYSTEMATIC REVIEW OF BIOACTIVE ALKALOIDS AND CLINICAL APPLICATIONS
DOI:
https://doi.org/10.53555/bp.v11i1.2475Keywords:
Aloe species, bioactive alkaloids, pharmacological properties, antioxidant, anti-inflammatoryAbstract
Background: Members of the genus Aloe are well known in traditional and modern medici ne because of their health
promoting effects. Different bioactive alkaloids, including aloin, aloe-emodin, acemannan, and barbaloin were found to
be responsible for Aloe’s beneficial uses. This review systematically analyses the therapeutic effects of compounds derived
from Aloe, with a focus on their impact at the level of the physiological system, and offering a chronologic view of the
importance of the use of preparations derived from Aloe. The purpose of this review article is to discuss about the
phytochemical composition, pharmacological attributes, therapeutic uses of Aloe species. The Aloe research also looks
at how Aloe has progressed in research, with great emphasis put on Aloe's old.
Methods:A systematic review of the literature was performed according to the PRSIMA statement. Journal articles
published in peer-reviewed journals were obtained from the PubMed, SCOPUS, Web of Science, Google Scholar, and
Science Direct. The inclusion criteria covered papers on Aloe phytochemistry, pharmacological properties, and
applications in therapy, and the exclusion criteria were non-peer-reviewed papers, and non-medicinal uses of Aloe.
Results: Aloe species have been reported to possess pharmacological properties such as anti-inflammatory, antimicrobial,
anti-oxidant, anti-diabetic, hepatoprotective, and wound healing activities. Chronological examination shows an
unbroken thread of study on the medicinal potential of Aloe, with early works focusing on its laxative and dermatological
capacities, and more recent studies supporting its effects on metabolism and neurological health..
Conclusion: Aloe species have been known for the wide therapeutic application as the potential source of bioactive
compounds. But differences in the phytochemical composition between them (including how they are grown) mean that
more research is needed as there have been few human trials Future studies should focus on optimizing Aloe formulations,
enhancing
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References
Khajeeyan, R., Salehi, A., Movahhedi Dehnavi, M., Hamidian, M., & Hazrati, S. (2024). Evaluation of the benefits
of plant growth-promoting rhizobacteria and mycorrhizal fungi on biochemical and morphophysiological traits of
Aloe barbadensis Mill under water deficit stress. Scientific reports, 14(1), 14480. https://doi.org/10.1038/s41598-
-64878-9
Aldayel, T. S., Badran, M. M., Alomrani, A. H., AlFaris, N. A., Altamimi, J. Z., Alqahtani, A. S., Nasr, F. A., Ghaffar,
S., & Orfali, R. (2023). Optimization of cationic nanoparticles stabilized by poloxamer 188: A potential approach
for improving the biological activity of Aloeperryi. Heliyon, 9(12), e22691.
https://doi.org/10.1016/j.heliyon.2023.e22691
Wang, X., Jin, Z., Mao, L., Tu, L., Sun, Y., & Tao, J. (2022). Study on the Skin Hydration and Trans Epidermal Water
Loss of Aloe Viscose Seamless Knitted Fabric for Autumn and Winter. Materials (Basel, Switzerland), 16(1), 212.
https://doi.org/10.3390/ma16010212
Bultum, L. E., Tolossa, G. B., Kim, G., Kwon, O., & Lee, D. (2022). In silico activity and ADMET profiling of
phytochemicals from Ethiopian indigenous aloes using pharmacophore models. Scientific reports, 12(1), 22221.
https://doi.org/10.1038/s41598-022-26446-x
Razia, S., Park, H., Shin, E., Shim, K. S., Cho, E., Kang, M. C., & Kim, S. Y. (2022). Synergistic effect of Aloe vera
flower and Aloe gel on cutaneous wound healing targeting MFAP4 and its associated signaling pathway: In-vitro
study. Journal of ethnopharmacology, 290, 115096. https://doi.org/10.1016/j.jep.2022.115096
Zhu, J. Z., Ma, Y. M., Wang, X. L., Zhong, J., Zhang, Z., & Li, X. G. (2022). First Report of Southern Blight on Aloe
vera Caused by Athelia rolfsii in China. Plant disease, 10.1094/PDIS-08-21-1850-PDN. Advance online publication.
https://doi.org/10.1094/PDIS-08-21-1850-PDN
Amare, G. G., Meharie, B. G., & Belayneh, Y. M. (2020). Evaluation of Antidiabetic Activity of the Leaf Latex
of Aloe pulcherrima Gilbert and Sebsebe (Aloaceae). Evidence-based complementary and alternative medicine :
eCAM, 2020, 8899743. https://doi.org/10.1155/2020/8899743
Teka, T., Awgichew, T., & Kassahun, H. (2020). Antimalarial Activity of the Leaf Latex of Aloe weloensis (Aloaceae)
against Plasmodium berghei in Mice. Journal of tropical medicine, 2020, 1397043.
https://doi.org/10.1155/2020/1397043
Penmetsa, G. S., & Pitta, S. R. (2019). Efficacy of Ocimum sanctum, Aloe vera and chlorhexidine mouthwash on
gingivitis: A randomized controlled comparative clinical study. Ayu, 40(1), 23–26.
https://doi.org/10.4103/ayu.AYU_212_18
Raj, B., Ingole, N., & Ambad, R. (2024). Evaluation of the Wound Healing Potential of Aloe Vera Leaf Extract
Containing Carbopol 934 as a Gelling Agent and Comparing with Sodium CMC and HPMC on Surgically Induced
in Wound Wister Rats: A Preclinical Study. Journal of pharmacy & bioallied sciences, 16(Suppl 4), S3699–S3702.
https://doi.org/10.4103/jpbs.jpbs_1101_24
Maan, S. A., Faiesal, A. A., Gamar, G. M., & El Dougdoug, N. K. (2025). Efficacy of bacteriophages with Aloe vera
extract in formulated cosmetics to combat multidrug-resistant bacteria in skin diseases. Scientific reports, 15(1),
https://doi.org/10.1038/s41598-025-86334-y
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Moharram, B. A., Alburyhi, M. M., Al-Maqtari, T., & Faisal, A. (2025). Evaluating the Antidiabetic Activity of Aloe
niebuhriana Latex in Alloxan-Induced Diabetic Rats and the Development of a Novel Effervescent Granule-Based
Delivery System. TheScientificWorldJournal, 2025, 5648662. https://doi.org/10.1155/tswj/5648662
Kebriti, I., Solgi, M., & Velashjerdi, M. (2025). Improving quality of strawberry by novel essential oil nanoemulsions
of Echinophora platyloba combined with Aloe vera gel and gum arabic. Scientific reports, 15(1), 1731.
https://doi.org/10.1038/s41598-025-86259-6
Triantafyllou, E., Karydis-Messinis, A., Moschovas, D., Kyriakaki, C., Vasilopoulos, K. C., Giannakas, A. E.,
Karakassides, M. A., Avgeropoulos, A., Zafeiropoulos, N. E., & Salmas, C. E. (2024). Microwave-Assisted
Extraction of Cellulose from Aloe Vera Plant Residue and Preparation of Cellulose Nanocrystal-Poly(vinyl alcohol)
Hydrogels. Molecules (Basel, Switzerland), 29(24), 6012. https://doi.org/10.3390/molecules29246012
Dayoob, M., Kee, Y. W. Y., Bahrin, W. H., Alrimawi, B. H., Kumarasamy, V., Subramaniyan, V., Albadrani, G. M.,
Al-Ghadi, M. Q., Kamel, M., Abdel-Daim, M. M., & Azad, A. K. (2024). Development and Characterization of
Fusidic Acid-Loaded Alginate-Aloe vera Based Hydrogel Film. Journal of visualized experiments : JoVE, (214),
3791/67068. https://doi.org/10.3791/67068
Hakami, Z. H., Abdo, W., Nazeam, J. A., Osman, S. M., Goda, W., Fadl, S. E., Alsulimani, A., Al-Noshokaty, T. M.,
Haridy, M., Alnasser, S. M., & Abdeen, A. (2025). Aloe arborescens Standardized Glycosidic Fraction Suppresses
Hepatocarcinoma by Modulating TIMP1, MMP9 Genes Expression, and Inflammation/Ki67/TGFβ1
Pathway. Phytotherapy research : PTR, 39(2), 1090–1106. https://doi.org/10.1002/ptr.8412
Wani, M. J., Zofair, S. F. F., Salman, K. A., Moin, S., & Hasan, A. (2025). Aloin reduces advanced glycation end
products, decreases oxidative stress, and enhances structural stability in glycated low-density
lipoprotein. International journal of biological macromolecules, 289, 138823.
https://doi.org/10.1016/j.ijbiomac.2024.138823
Feroze, F., Sher, M., Hussain, M. A., Abbas, A., Haseeb, M. T., Fatima, A., Naeem-Ul-Hassan, M., & Amin, H. M.
A. (2025). Gastro retentive floating drug delivery system of levofloxacin based on Aloe vera hydrogel: In vitro and
in vivo assays. International journal of biological macromolecules, 284(Pt 1), 138156.
https://doi.org/10.1016/j.ijbiomac.2024.138156
Calzoni, E., Bertoldi, A., Cesaretti, A., Alabed, H. B. R., Cerrotti, G., Pellegrino, R. M., Buratta, S., Urbanelli, L., &
Emiliani, C. (2024). Aloe Extracellular Vesicles as Carriers of Photoinducible Metabolites Exhibiting Cellular
Phototoxicity. Cells, 13(22), 1845. https://doi.org/10.3390/cells13221845
Shih, P. C., Lin, C. H., Chokkalingam, U., Prakash, E., Kao, C. N., Chang, C. F., & Lin, W. L. (2024). The Aloe vera
acemannan polysaccharides inhibit phthalate-induced cell viability, metastasis, and stemness in colorectal cancer
cells. Ecotoxicology and environmental safety, 288, 117351. https://doi.org/10.1016/j.ecoenv.2024.117351
Wang, X., Yang, J., Zhao, Q., Xie, X., Deng, F., Wang, Z., Jiang, K., Li, X., Liu, H., Shi, Z., Zhu, X., Chen, L., &
Lv, D. (2024). A tissue-adhesive, mechanically enhanced, natural Aloe Vera-based injectable hydrogel for wound
healing: Macrophage mediation and collagen proliferation. International journal of biological
macromolecules, 283(Pt 1), 137452. https://doi.org/10.1016/j.ijbiomac.2024.137452
Ouyang, F., Li, Y., Wang, H., Liu, X., Tan, X., Xie, G., Zeng, J., Zeng, G., Luo, Q., Zhou, H., Chen, S., Hou, K.,
Fang, J., Zhang, X., Zhou, L., Li, Y., & Gao, A. (2024). Aloe Emodin Alleviates Radiation-Induced Heart Disease
via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption. Advanced science (Weinheim,
Baden-Wurttemberg, Germany), 11(47), e2406026. https://doi.org/10.1002/advs.202406026
Sulayman, R., Ali, S., Ullah, R., Summer, M., Hassan, A., Shahzad, H., Fiaz, U., Mumtaz, S., Nauroze, T., & Mumtaz,
S. (2024). A comprehensive study on mechanisms of action of fibroin, aloe vera, and ginger extracts through
histochemical, inflammation biomarkers, and matrix metalloproteinases analysis against diabetic wounds. Journal
of tissue viability, 33(4), 949–959. https://doi.org/10.1016/j.jtv.2024.10.006
Romero-Alemán, M. D., Pérez-Galván, J. M., Hernández-Rodríguez, J. E., & Monzón-Mayor, M. (2025). The
Potential of Aloe Vera in Solution and in Blended Nanofibers Containing Poly (3-Hydroxybutyrate-Co-3-
Hydroxyvalerate) as Substrates for Neurite Outgrowth. Journal of biomedical materials research. Part A, 113(1),
e37825. https://doi.org/10.1002/jbm.a.37825
Shetty, A. P., Halemani, K., Issac, A., Thimmappa, L., Shashidhara, Y. N., Mathias, E., Dhiraaj, S., Radha, K., Mishra,
P., & Sharma, A. (2024). Effectiveness of the Application of Lanolin, Aloe Vera, and Peppermint on Nipple Pain and
Nipple Trauma in Lactating Mothers: A Systematic Review and Meta-Analysis. Maternal and child health
journal, 28(12), 2013–2025. https://doi.org/10.1007/s10995-024-04006-1
Chen, S., Liao, T., Zhao, S., Wang, B., Yu, L., Jiang, T., Hao, T., Wu, W., Li, C., Shen, F., & Zhang, Q. (2024). High
strength "breathable" glycosilicone/Aloe vera polysaccharide-based gel dressing for efficient wound
repair. International journal of biological macromolecules, 281(Pt 4), 136293.
https://doi.org/10.1016/j.ijbiomac.2024.136293
Singh, S., Kumar, S., & Dhanasingh, I. (2024). Overexpression, purification and biochemical studies of Sortase A
from Enterococcus faecalis (Ef) and its inhibition studies with Aloenin. Acta tropica, 260, 107419.
https://doi.org/10.1016/j.actatropica.2024.107419
Zhao, Y., Zhang, T., Liang, Y., Xie, X., Pan, H., Cao, M., Wang, S., Wu, D., Wang, J., Wang, C., & Hu, W. (2024).
Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter
baumannii to polymyxins. Frontiers in cellular and infection microbiology, 14, 1467607.
https://doi.org/10.3389/fcimb.2024.1467607
Subramaniyan, G., Rubina, S., Ramana, B. V., Stanley, A. M., & Srinivasan, D. (2024). Aloe vera leaf mucilage and
lemon oil as potential penetration-enhancing agents to increase lornoxicam transdermal administration using nano
vesicular gel. Pakistan journal of pharmaceutical sciences, 37(3), 499–509.
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Shen, X., Gong, L., Li, R., Huang, N., Zhang, H., Chen, S., Liu, Y., & Sun, R. (2024). Treatment of constipation
with Aloe and its compatibility prescriptions. Chinese herbal medicines, 16(4), 561–571.
https://doi.org/10.1016/j.chmed.2024.07.005
Shih, P. C., Lin, C. H., Chokkalingam, U., Prakash, E., Kao, C. N., Chang, C. F., & Lin, W. L. (2024). The Aloe vera
acemannan polysaccharides inhibit phthalate-induced cell viability, metastasis, and stemness in colorectal cancer
cells. Ecotoxicology and environmental safety, 288, 117351. https://doi.org/10.1016/j.ecoenv.2024.117351
Chen, R., Zhang, H., Zhao, X., Zhu, L., Zhang, X., Ma, Y., & Xia, L. (2024). Progress on the mechanism of action
of emodin against breast cancer cells. Heliyon, 10(21), e38628. https://doi.org/10.1016/j.heliyon.2024.e38628
Ouyang, F., Li, Y., Wang, H., Liu, X., Tan, X., Xie, G., Zeng, J., Zeng, G., Luo, Q., Zhou, H., Chen, S., Hou, K.,
Fang, J., Zhang, X., Zhou, L., Li, Y., & Gao, A. (2024). Aloe Emodin Alleviates Radiation-Induced Heart Disease
via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption. Advanced science (Weinheim,
Baden-Wurttemberg, Germany), 11(47), e2406026. https://doi.org/10.1002/advs.202406026
Wang, X., Chen, H., Shao, N., Zhang, X., Huang, C., Li, X., Zhang, J., Chang, Z., Tang, L., & Xie, D. (2024).
Protective Effect of Aloe-emodin on Cognitive Function in Copper-loaded Rats Based on The Inhibition of
Hippocampal Neuron Ferroptosis. Current neurovascular research, 10.2174/0115672026348862241003042336.
Advance online publication. https://doi.org/10.2174/0115672026348862241003042336
Zhao, Y., Zhang, T., Liang, Y., Xie, X., Pan, H., Cao, M., Wang, S., Wu, D., Wang, J., Wang, C., & Hu, W. (2024).
Combination of aloe emodin, emodin, and rhein from Aloe with EDTA sensitizes the resistant Acinetobacter
baumannii to polymyxins. Frontiers in cellular and infection microbiology, 14, 1467607.
https://doi.org/10.3389/fcimb.2024.1467607
Elkomy, N. M. I. M., El-Shaibany, A., Al-Mahbashi, H., Abdelkhalek, A. S., Elnagar, G. M., Elaasser, M. M., &
Raslan, A. E. (2025). Evaluation of in-vitro antioxidant activity, acute oral toxicity, and pancreatic and hepatic
protective effects of Aloe rubroviolacea flowers extract against CCl4 toxicity in a rat model. Journal of
ethnopharmacology, 337(Pt 1), 118768. https://doi.org/10.1016/j.jep.2024.118768
Yang, Y., Sun, J., You, H., Sun, Y., Song, Y., Shen, Z., Liu, T., Guan, D., Zhou, Y., Cheng, S., Wang, C., Yu, G., Zhu,
C., & Tang, Z. (2024). Aloe-emodin relieves allergic contact dermatitis pruritus by inhibiting mast cell
degranulation. Immunology letters, 270, 106902. https://doi.org/10.1016/j.imlet.2024.106902
Yang, D., Ge, T., Zhou, J., Li, H., & Zhang, Y. (2024). Aloe-emodin alleviates inflammatory bowel disease in mice
by modulating intestinal microbiome homeostasis via the IL-4/IL-13 axis. Heliyon, 10(15), e34932.
https://doi.org/10.1016/j.heliyon.2024.e34932
Syraji, Y., Kebebew, M., Techane, Y., & Albene, D. (2024). Antimicrobial, Antiradical Activity, and X-Ray
Fluorescence Spectroscopy Analysis of Aloe otallensis Plant Used in Traditional Medicine in Southern
Ethiopia. International journal of microbiology, 2024, 1981990. https://doi.org/10.1155/2024/1981990
Patitucci, F., Motta, M. F., Mileti, O., Dattilo, M., Malivindi, R., Pezzi, G., Gabriele, D., Parisi, O. I., & Puoci, F.
(2025). Enhancing burn wound care with pre-crosslinked 3D-printed patches: Bromelain delivery and aloe vera
bioactives integration for improved healing outcomes. International journal of pharmaceutics, 672, 125304.
Advance online publication. https://doi.org/10.1016/j.ijpharm.2025.125304
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Carballo-López, G. I., Ojeda-González, J., Martínez-García, K. D., Cervantes-Luevano, K. E., Moreno-Ulloa, A., &
Castro-Ceseña, A. B. (2025). Enhanced anti-inflammatory and anti-fibrotic effects of nanoparticles loaded with a
combination of Aloe vera-Moringa oleifera extracts. Molecular omics, 10.1039/d4mo00195h. Advance online
publication. https://doi.org/10.1039/d4mo00195h
Ghosh, I., Banerjee, S., Dutta, S., Maji, H. S., & Mondal, A. (2025). Exploring the anti-diabetic potential of Aloe
vera: isolation and characterization of bioactive compounds. Natural product research, 1–7. Advance online
publication. https://doi.org/10.1080/14786419.2025.2451775
Pal, S., Raj, M., Singh, M., Saurav, K., Paliwal, C., Saha, S., Sharma, A. K., & Singh, M. (2024). The Effect of Aloe
vera on Skin and Its Commensals: Contribution of Acemannan in Curing Acne Caused by Propionibacterium
acnes. Microorganisms, 12(10), 2070. https://doi.org/10.3390/microorganisms12102070
Gutiérrez Rafael, B. J., Zaca Moran, O., Delgado Macuil, R. J., Martínez Gutiérrez, H., García Juárez, M., & Lopez
Gayou, V. (2024). Study of the Incorporation of Gel and Aloe vera Peel Extract in a Polymer Matrix Based on
Polyvinylpyrrolidone. Polymers, 16(14), 1998. https://doi.org/10.3390/polym16141998
Edo G. I. (2024). Coordination of bioactive phytochemicals from Aloe vera extracts to metal ions; investigation of
the metal complexes and bioactive compound formed. Biometals : an international journal on the role of metal ions
in biology, biochemistry, and medicine, 37(6), 1379–1391. https://doi.org/10.1007/s10534-024-00611-3
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Moharram, B. A., Alburyhi, M. M., Al-Maqtari, T., & Faisal, A. (2025). Evaluating the Antidiabetic Activity of Aloe
niebuhriana Latex in Alloxan-Induced Diabetic Rats and the Development of a Novel Effervescent Granule-Based
Delivery System. TheScientificWorldJournal, 2025, 5648662. https://doi.org/10.1155/tswj/5648662
Shen, X., Gong, L., Li, R., Huang, N., Zhang, H., Chen, S., Liu, Y., & Sun, R. (2024). Treatment of constipation
with Aloe and its compatibility prescriptions. Chinese herbal medicines, 16(4), 561–571.
https://doi.org/10.1016/j.chmed.2024.07.005
Chen, S., Dou, Y., Zhang, S., Qiu, H., Liu, H., Xu, Y., Shu, L., & Li, Y. (2025). Rapid Identification of Chemical
Constituents in New Compound Aloe Capsule Based on UPLC-Q-Orbitrap-MS. Rapid communications in mass
spectrometry : RCM, 39(3), e9944. https://doi.org/10.1002/rcm.9944
Shih, P. C., Lin, C. H., Chokkalingam, U., Prakash, E., Kao, C. N., Chang, C. F., & Lin, W. L. (2024). The Aloe vera
acemannan polysaccharides inhibit phthalate-induced cell viability, metastasis, and stemness in colorectal cancer
cells. Ecotoxicology and environmental safety, 288, 117351. https://doi.org/10.1016/j.ecoenv.2024.117351
Melenshia, D. S., Amirtham, S. M., Rebekah, G., Vinod, E., & Kachroo, U. (2024). Effect of reconstituted,
lyophilized cold aqueous extract of Aloe vera on human whole blood clotting time - A pilot study. Journal of
Ayurveda and integrative medicine, 15(2), 100887. https://doi.org/10.1016/j.jaim.2024.100887
Abdullah, Z., Ashraf, M. U., Barkat, K., Badshah, S. F., Rehman, U., Razzaq, A., Mahmood, A., Ulhaq, F., Chopra,
H., Rashid, S., Valko, M., Alomar, S., Kuca, K., & Sharma, R. (2023). Formulation of pH-responsive highly swellable
hydrogel scaffolds for controlled release of tramadol HCl: characterization and biocompatibility
evaluation. Frontiers in bioengineering and biotechnology, 11, 1190322.
https://doi.org/10.3389/fbioe.2023.1190322
Bohra, A., Maheswari, T. N. U., Harsh, A., & Garg, A. (2021). Black Turmeric and Aloe Vera in the Management of
Oral Submucous Fibrosis: A Prospective Clinical Study. Asian Pacific journal of cancer prevention : APJCP, 22(12),
–3947. https://doi.org/10.31557/APJCP.2021.22.12.3941
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Shen, X., Gong, L., Li, R., Huang, N., Zhang, H., Chen, S., Liu, Y., & Sun, R. (2024). Treatment of constipation
with Aloe and its compatibility prescriptions. Chinese herbal medicines, 16(4), 561–571.
https://doi.org/10.1016/j.chmed.2024.07.005
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Liao, B., Zhang, C., Shen, J., Chen, D., Wang, J., Chen, X., Zhou, Y., Wei, Y., Shi, Y., Gou, L., Guo, Q., Zhou, X.,
Xie, H., Zhao, L., Liao, G., Zhu, Z., Cheng, L., Zhou, X., Li, Y., & Ren, B. (2025). Aloin remodels the cell wall of
Candida albicans to reduce its hyphal virulence against oral candidiasis. Applied microbiology and
biotechnology, 109(1), 21. https://doi.org/10.1007/s00253-025-13411-7
Shih, P. C., Lin, C. H., Chokkalingam, U., Prakash, E., Kao, C. N., Chang, C. F., & Lin, W. L. (2024). The Aloe vera
acemannan polysaccharides inhibit phthalate-induced cell viability, metastasis, and stemness in colorectal cancer
cells. Ecotoxicology and environmental safety, 288, 117351. https://doi.org/10.1016/j.ecoenv.2024.117351
Ouyang, F., Li, Y., Wang, H., Liu, X., Tan, X., Xie, G., Zeng, J., Zeng, G., Luo, Q., Zhou, H., Chen, S., Hou, K.,
Fang, J., Zhang, X., Zhou, L., Li, Y., & Gao, A. (2024). Aloe Emodin Alleviates Radiation-Induced Heart Disease
via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption. Advanced science (Weinheim,
Baden-Wurttemberg, Germany), 11(47), e2406026. https://doi.org/10.1002/advs.202406026
Tornero-Martínez, A., Cruz-Ortiz, R., Jaramillo-Flores, M. E., Osorio-Díaz, P., Ávila-Reyes, S. V., Alvarado-Jasso,
G. M., & Mora-Escobedo, R. (2019). In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of
Antioxidant Activity and Production of Short Chain Fatty Acids. Molecules (Basel, Switzerland), 24(19), 3605.
https://doi.org/10.3390/molecules24193605
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Carballo-López, G. I., Ojeda-González, J., Martínez-García, K. D., Cervantes-Luevano, K. E., Moreno-Ulloa, A., &
Castro-Ceseña, A. B. (2025). Enhanced anti-inflammatory and anti-fibrotic effects of nanoparticles loaded with a
combination of Aloe vera-Moringa oleifera extracts. Molecular omics, 10.1039/d4mo00195h. Advance online
publication. https://doi.org/10.1039/d4mo00195h
Krupa, S., Ruman, T., Szuberla, W., & Nizioł, J. (2025). Analysis of the spatial distribution of metabolites in Aloe
vera leaves by mass spectrometry imaging and UHPLC-UHRMS. Scientific reports, 15(1), 3502.
https://doi.org/10.1038/s41598-025-88144-8
Ghosh, I., Banerjee, S., Dutta, S., Maji, H. S., & Mondal, A. (2025). Exploring the anti-diabetic potential of Aloe
vera: isolation and characterization of bioactive compounds. Natural product research, 1–7. Advance online
publication. https://doi.org/10.1080/14786419.2025.2451775
Iosageanu, A., Mihai, E., Seciu-Grama, A. M., Utoiu, E., Gaspar-Pintiliescu, A., Gatea, F., Cimpean, A., &
Craciunescu, O. (2024). In Vitro Wound-Healing Potential of Phenolic and Polysaccharide Extracts of Aloe
vera Gel. Journal of functional biomaterials, 15(9), 266. https://doi.org/10.3390/jfb15090266
Elkomy, N. M. I. M., El-Shaibany, A., Al-Mahbashi, H., Abdelkhalek, A. S., Elnagar, G. M., Elaasser, M. M., &
Raslan, A. E. (2025). Evaluation of in-vitro antioxidant activity, acute oral toxicity, and pancreatic and hepatic
protective effects of Aloe rubroviolacea flowers extract against CCl4 toxicity in a rat model. Journal of
ethnopharmacology, 337(Pt 1), 118768. https://doi.org/10.1016/j.jep.2024.118768
Pimpa, J., Authaida, S., Boonkum, W., Rerkyusuke, S., Janta, C., & Chankitisakul, V. (2024). Unveiling the Potential
of Aloe vera Gel Supplementation in a Cooling Extender: A Breakthrough in Enhancing Rooster Sperm Quality and
Fertility Ability. Animals : an open access journal from MDPI, 14(16), 2290. https://doi.org/10.3390/ani14162290
Hotan, O., Alhaj, A., Al-Quhaim, A., Alburaihi, K., Ahmed, Y., Munasser, Q., Dhufer, S. B., Nasran, T., Gabir, M.,
Ebrahim, A., Obadi, M., Hadi, M., Al-Baity, H., Ba-Nafea, A., Qaed, E., Zaky, M. Y., Okba, M., Al-Nasi, A., &
Almoiliqy, M. (2024). Evaluating the pharmacological activities of Aloe perryi-Silver nanoparticles induced
apoptosis against colon cancer cells (HCT-116). Food science & nutrition, 12(8), 5890–5906.
https://doi.org/10.1002/fsn3.4246
Yoruk, N. G., & Istanbullu Paksoy, Ö. (2024). GC/MS evaluation of the composition of the Aloe vera gel and
extract. Food chemistry: X, 23, 101536. https://doi.org/10.1016/j.fochx.2024.101536
Khajeeyan, R., Salehi, A., Movahhedi Dehnavi, M., Hamidian, M., & Hazrati, S. (2024). Evaluation of the benefits
of plant growth-promoting rhizobacteria and mycorrhizal fungi on biochemical and morphophysiological traits of
Aloe barbadensis Mill under water deficit stress. Scientific reports, 14(1), 14480. https://doi.org/10.1038/s41598-
-64878-9
Yang, X., Li, C., Ge, M., Li, X., Zhao, W., Guo, H., Nie, H., & Liu, J. (2024). Mn(II)-Aloe-Emodin Nanoscale
Coordination Polymer Enhances Ferroptosis by Synergistically Enhancing Reactive Oxygen Species Generation via
the Nrf2-GPX4 Axis. Advanced healthcare materials, 13(28), e2400474. https://doi.org/10.1002/adhm.202400474
Pal, D., Mitra, D., Hazra, S., & Ghosh, D. (2024). Protective Effect of Aloe vera (L.) on Diabetes-Induced Oxidative
Stress Linked Spermiological Co-Morbidity in Human and Rat: An In-Vitro Analysis. Reproductive sciences
(Thousand Oaks, Calif.), 31(9), 2685–2699. https://doi.org/10.1007/s43032-024-01605-9
Khurshid, S., Farwa, U., Mazhar, Z., Naeem, H., & Ishaq, Y. (2024). Evaluation of Aloe Vera Gel Combined with
Cinnamon Oil with Antibacterial Activity for Wound Healing Application. The international journal of lower
extremity wounds, 15347346241259102. Advance online publication. https://doi.org/10.1177/15347346241259102
Getahun, T., Das, J., Sil, P. C., & Gupta, N. (2024). Antibacterial and Antioxidant Compounds from the Root Extract
of Aloe debrana. Evidence-based complementary and alternative medicine : eCAM, 2024, 6651648.
https://doi.org/10.1155/2024/6651648
Sharma, D., Sharma, A., Bala, R., & Singh, B. (2024). Investigations on physiochemical and biomedical properties
of Aloe vera - Sterculia gum copolymeric dressings impregnated with antibiotic-anesthetic drugs to enhance wound
healing. International journal of biological macromolecules, 267(Pt 2), 131363.
https://doi.org/10.1016/j.ijbiomac.2024.131363
Girgin, R., Çeker, G., Turan, I., Kutu, F., Elma, Y., & Yılmaz Can, E. (2024). The effects of topical aloe vera on an
experimentally designed penile fracture model in rats. Sıçanlarda deneysel olarak tasarlanmış penil fraktur modelinde
topikal aloe veranın etkileri. Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery:
TJTES, 30(3), 147–154. https://doi.org/10.14744/tjtes.2024.26425
Komal, Shabaan, M., Ali, Q., Asghar, H. N., Zahir, Z. A., Yousaf, K., Aslam, N., Zulfiqar, U., Ejaz, M., Alwahibi,
M. S., & Ali, M. A. (2024). Exploring the synergistic effect of chromium (Cr) tolerant Pseudomonas aeruginosa and
nano zero valent iron (nZVI) for suppressing Cr uptake in Aloe Vera. International journal of
phytoremediation, 26(9), 1474–1485. https://doi.org/10.1080/15226514.2024.2327838
Hassan, R. E., Saleh, E. M., & Hamdy, G. M. (2025). Aloe vera gel relieves cadmium triggered hepatic injury via
antioxidative, anti-inflammatory, and anti-apoptotic routes. Biological trace element research, 203(1), 218–228.
https://doi.org/10.1007/s12011-024-04141-4
Mohamed, A. O., Abd-Elghaffar, S. K., Mousa, R. A., & Kamel, A. A. (2024). Aloe vera gel confers therapeutic
effect by reducing pyroptosis in ethanol-induced gastric ulcer rat model: Role of NLRP3/GSDMD signaling
pathway. Molecular biology reports, 51(1), 401. https://doi.org/10.1007/s11033-024-09329-4
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Triantafyllou, E., Karydis-Messinis, A., Moschovas, D., Kyriakaki, C., Vasilopoulos, K. C., Giannakas, A. E.,
Karakassides, M. A., Avgeropoulos, A., Zafeiropoulos, N. E., & Salmas, C. E. (2024). Microwave-Assisted
Extraction of Cellulose from Aloe Vera Plant Residue and Preparation of Cellulose Nanocrystal-Poly(vinyl alcohol)
Hydrogels. Molecules (Basel, Switzerland), 29(24), 6012. https://doi.org/10.3390/molecules29246012
Saenchoopa, A., Plaeyao, K., Talodthaisong, C., Thet Tun, W. S., Nasomjai, P., Lapmanee, S., Somsakeesit, L. O.,
Hutchison, J. A., & Kulchat, S. (2025). Development of Antibacterial Hydrogels Based on Biopolymer Aloe
Vera/Gelatin/Sodium Alginate Composited With SM-AgNPs Loaded Curcumin-Nanoliposomes. Macromolecular
bioscience, e2400504. Advance online publication. https://doi.org/10.1002/mabi.202400504
Gül, D., Özdemir, S. A., Yücel, O., Yıldırım, E., Kalyon, G., Sungur, E., Emik, S., Erol, A., & Kara, N. T. (2024).
Antimicrobial and Antibiofilm Activity of Green Synthesized Silver Nanoparticles by Using In Vitro Grown Aloe
vera L. Microscopy research and technique, 10.1002/jemt.24768. Advance online publication.
https://doi.org/10.1002/jemt.24768
Plascencia Martinez, D. F., Quiroz Castillo, J. M., Ospina Orejarena, A., Pérez Gallardo, A., Méndez Merino, E.,
Trimmer López, G. A., López Peña, I. Y., Hernández Martínez, D., López Gastelum, K. A., Leyva Verduzco, A. A.,
Ledezma, A. S., & Castillo Ortega, M. M. (2024). Comparative Study of Single and Coaxial Electrospun
Antimicrobial Cross-Linked Scaffolds Enriched with Aloe Vera: Characterization, Antimicrobial Activity, Drug
Delivery, Cytotoxicity, and Cell Proliferation on Adipose Stem Cells and Human Skin Fibroblast. ACS omega, 9(40),
–41170. https://doi.org/10.1021/acsomega.4c01016
Iosageanu, A., Mihai, E., Seciu-Grama, A. M., Utoiu, E., Gaspar-Pintiliescu, A., Gatea, F., Cimpean, A., &
Craciunescu, O. (2024). In Vitro Wound-Healing Potential of Phenolic and Polysaccharide Extracts of Aloe
vera Gel. Journal of functional biomaterials, 15(9), 266. https://doi.org/10.3390/jfb15090266
Seneviratne, D. M., Raphael, B., Whiteside, E. J., Windus, L. C. E., Kauter, K., Dearnaley, J. D. W., Annamalai, P.
K., Ward, R., Song, P., & Burey, P. P. (2024). A low-cost, antimicrobial aloe-alginate hydrogel film containing
Australian First Nations remedy 'lemon myrtle oil' (Backhousia citriodora) - Potential for incorporation into wound
dressings. Heliyon, 10(18), e37516. https://doi.org/10.1016/j.heliyon.2024.e37516
Velázquez-López, A. A., Torres-Gregorio, M., García-Rojas, V., Hernández-Sánchez, H., & Mora-Escobedo, R.
(2025). In Vitro Study of the Biological Activity of Aloe barbadensis Miller and its Physicochemical
Characterization. Plant foods for human nutrition (Dordrecht, Netherlands), 80(1), 55.
https://doi.org/10.1007/s11130-025-01299-7
Carballo-López, G. I., Ojeda-González, J., Martínez-García, K. D., Cervantes-Luevano, K. E., Moreno-Ulloa, A., &
Castro-Ceseña, A. B. (2025). Enhanced anti-inflammatory and anti-fibrotic effects of nanoparticles loaded with a
combination of Aloe vera-Moringa oleifera extracts. Molecular omics, 10.1039/d4mo00195h. Advance online
publication. https://doi.org/10.1039/d4mo00195h
Saenchoopa, A., Plaeyao, K., Talodthaisong, C., Thet Tun, W. S., Nasomjai, P., Lapmanee, S., Somsakeesit, L. O.,
Hutchison, J. A., & Kulchat, S. (2025). Development of Antibacterial Hydrogels Based on Biopolymer Aloe
Vera/Gelatin/Sodium Alginate Composited With SM-AgNPs Loaded Curcumin-Nanoliposomes. Macromolecular
bioscience, e2400504. Advance online publication. https://doi.org/10.1002/mabi.202400504
Wani, M. J., Zofair, S. F. F., Salman, K. A., Moin, S., & Hasan, A. (2025). Aloin reduces advanced glycation end
products, decreases oxidative stress, and enhances structural stability in glycated low-density
lipoprotein. International journal of biological macromolecules, 289, 138823.
https://doi.org/10.1016/j.ijbiomac.2024.138823
Shih, P. C., Lin, C. H., Chokkalingam, U., Prakash, E., Kao, C. N., Chang, C. F., & Lin, W. L. (2024). The Aloe vera
acemannan polysaccharides inhibit phthalate-induced cell viability, metastasis, and stemness in colorectal cancer
cells. Ecotoxicology and environmental safety, 288, 117351. https://doi.org/10.1016/j.ecoenv.2024.117351
Ren, Q., Chen, J., Wesseling, S., Bouwmeester, H., & Rietjens, I. M. C. M. (2024). Physiologically based Kinetic
Modeling-Facilitated Quantitative In Vitro to In Vivo Extrapolation to Predict the Effects of Aloe-Emodin in Rats
and Humans. Journal of agricultural and food chemistry, 72(29), 16163–16176.
https://doi.org/10.1021/acs.jafc.4c00969
Shin, B., Hillyer, T., & Shin, W. S. (2024). Rational Design and Testing of Antibacterial Aloe Vera Hemostatic
Hydrogel. Gels (Basel, Switzerland), 10(6), 409. https://doi.org/10.3390/gels10060409
Hayes, A. W., Pressman, P., Clemens, R., Singer, A. W., & Bauter, M. R. (2024). Evaluation of 90-day repeated dose
oral toxicity of an aloe vera inner leaf gel beverage. Food and chemical toxicology : an international journal
published for the British Industrial Biological Research Association, 189, 114726.
https://doi.org/10.1016/j.fct.2024.114726
Hassan, R. E., Saleh, E. M., & Hamdy, G. M. (2025). Aloe vera gel relieves cadmium triggered hepatic injury via
antioxidative, anti-inflammatory, and anti-apoptotic routes. Biological trace element research, 203(1), 218–228.
https://doi.org/10.1007/s12011-024-04141-4
Elkomy, N. M. I. M., El-Shaibany, A., Elnagar, G. M., Abdelkhalek, A. S., Al-Mahbashi, H., Elaasser, M. M., Raweh,
S. M., Aldiyarbi, M. A., & Raslan, A. E. (2023). Evaluation of acute oral toxicity, anti-diabetic and antioxidant effects
of Aloe vera flowers extract. Journal of ethnopharmacology, 309, 116310. https://doi.org/10.1016/j.jep.2023.116310
Ghosh, A., Saha, K., Bhattacharya, T., Sarkar, S., Sengupta, D., Maiti, A., Ghoshal, D., Dey, S., & Chattopadhyay,
D. (2024). Electrospun Cerium Oxide Nanoparticle/Aloe Vera Extract-Loaded Nanofibrous Poly(Ethylene
Oxide)/Polyurethane Mats As Diabetic Wound Dressings. ACS applied bio materials, 7(8), 5268–5278.
https://doi.org/10.1021/acsabm.4c00475
Ren, Q., Chen, J., Wesseling, S., Bouwmeester, H., & Rietjens, I. M. C. M. (2024). Physiologically based Kinetic
Modeling-Facilitated Quantitative In Vitro to In Vivo Extrapolation to Predict the Effects of Aloe-Emodin in Rats
and Humans. Journal of agricultural and food chemistry, 72(29), 16163–16176.
https://doi.org/10.1021/acs.jafc.4c00969
Nauroze, T., Ali, S., Andleeb, S., Ara, C., Liaqat, I., Mushtaq, H., Mumtaz, S., Kanwal, L., Abbas, A. S., Mumtaz,
S., Farooq, M. A., & Khan, I. H. (2024). Therapeutic Potential of Aloe vera and Aloe vera-Conjugated Silver
Nanoparticles on Mice Exposed to Hexavalent Chromium. Biological trace element research, 202(12), 5580–5595.
https://doi.org/10.1007/s12011-024-04105-8
Hassan, R. E., Saleh, E. M., & Hamdy, G. M. (2025). Aloe vera gel relieves cadmium triggered hepatic injury via
antioxidative, anti-inflammatory, and anti-apoptotic routes. Biological trace element research, 203(1), 218–228.
https://doi.org/10.1007/s12011-024-04141-4
Gupta, V. K., Park, U., Siddiqi, N. J., Huh, Y. S., & Sharma, B. (2023). Amelioration of Hepatotoxic and Neurotoxic
Effect of Cartap by Aloe vera in Wistar Rats. Toxics, 11(5), 472. https://doi.org/10.3390/toxics11050472
Kweon, D. Y., Song, H. J., Kim, J. E., Jin, Y. J., Roh, Y. J., Seol, A., Park, J. M., Lee, E. S., Choi, W. S., & Hwang,
D. Y. (2023). Therapeutic Effects of Aloe saponaria against Ulcerative Colitis Induced by Dextran Sulfate
Sodium. Current issues in molecular biology, 45(2), 1483–1499. https://doi.org/10.3390/cimb45020096
Hu, Y. H., Quan, Z. Y., Li, D. K., Wang, C. Y., & Sun, Z. X. (2022). Inhibition of CYP3A4 enhances aloe-emodin
induced hepatocyte injury. Toxicology in vitro : an international journal published in association with BIBRA, 79,
https://doi.org/10.1016/j.tiv.2021.105276
Hoogenboom, T. C. H., Patel, N., Cook, N. A., Williams, R., Taylor-Robinson, S. D., & Lim, A. K. P. (2020). The
Effect of Aloe Vera Juice on Liver Enzymes and Hepatic Structure in a Healthy Population. Integrative medicine
(Encinitas, Calif.), 19(3), 30–34.
Gupta, V. K., Kumar, A., Pereira, M. L., Siddiqi, N. J., & Sharma, B. (2020). Anti-Inflammatory and Antioxidative
Potential of Aloe vera on the Cartap and Malathion Mediated Toxicity in Wistar Rats. International journal of
environmental research and public health, 17(14), 5177. https://doi.org/10.3390/ijerph17145177
Liu, D. M., Yang, D., Zhou, C. Y., Wu, J. S., Zhang, G. L., Wang, P., Wang, F., & Meng, X. L. (2020). Aloe-emodin
induces hepatotoxicity by the inhibition of multidrug resistance protein 2. Phytomedicine : international journal of
phytotherapy and phytopharmacology, 68, 153148. https://doi.org/10.1016/j.phymed.2019.153148
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